Anti-Mouse Ly-6G BG Violet 500
Additional 1A8 Formats
| Cat.No. | Format | Starting From |
|---|---|---|
| 83112-87 | APC-Cyanine7 | $ 80.00 |
| 83112-50 | FITC | $ 60.00 |
| 83112-80 | APC | $ 70.00 |
| 83112-70 | PerCP-Cyanine5.5 | $ 85.00 |
| 83112-40 | BG Violet 450 | $ 85.00 |
| 83112-25 | SAFIRE Purified | $ 135.00 |
| 83112-60 | PE | $ 50.00 |
| 83112-77 | PE-Cyanine7 | $ 80.00 |
| 83112-45 | BG Violet 500 | $ 85.00 |
description
The 1A8 monoclonal antibody specifically reacts with the 21-25 kDa glycophosphatidylinositol-anchored protein known as Ly-6G, expressed by the granulocytes from the bone marrow and periphery neutrophils. Ly-6G and Ly-6C form the Granulocyte Receptor-1 antigen (GR-1).
The binding of the 1A8 antibody to the Ly-6G can be blocked by another antibody, RB6-8C5, which also recognizes Ly-6C. While 1A8 is specific for only Ly-6G, RB6-8C5 also binds to Ly-6C.
BG Violet 500 conjugate is an alternative to the Pacific Orange and BD Horizon V500 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 500nm.
Additional Information
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Clone:
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1A8
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Format:
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BG Violet 500
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Applications:
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FC
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Reactivity:
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Mouse
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Isotype:
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Rat IgG2a, kappa
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| Research Interest: | Adaptive Immunity |
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| Cell Type: | T Cells, NK and NKT Cells, Macrophage / Monocyte |
| Application: | FC |
| Clone: | 1A8 |
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Preparation:
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The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
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Formulation:
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Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
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References:
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Fleming, T. J., Fleming, M. L., & Malek, T. R. (1993). Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family.The Journal of Immunology, 151(5), 2399-2408. Daley, J. M., Thomay, A. A., Connolly, M. D., Reichner, J. S., & Albina, J. E. (2008). Use of Ly6G-specific monoclonal antibody to deplete neutrophils in mice. Journal of leukocyte biology, 83(1), 64-70. Dietlin, T. A., Hofman, F. M., Lund, B. T., Gilmore, W., Stohlman, S. A., & van der Veen, R. C. (2007). Mycobacteria-induced Gr-1+ subsets from distinct myeloid lineages have opposite effects on T cell expansion. Journal of leukocyte biology, 81(5), 1205-1212. |