Anti-Mouse Ly-6G BG Violet 450

Catalog Number : 83112-40

Description

The 1A8 monoclonal antibody specifically reacts with the 21-25 kDa glycophosphatidylinositol-anchored protein known as Ly-6G, expressed by the granulocytes from the bone marrow and periphery neutrophils. Ly-6G and Ly-6C form the Granulocyte Receptor-1 antigen (GR-1).

The binding of the 1A8 antibody to the Ly-6G can be blocked by another antibody, RB6-8C5, which also recognizes Ly-6C. While 1A8 is specific for only Ly-6G, RB6-8C5 also binds to Ly-6C.

BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.

Additional Information

Clone:	1A8
Format:	BG Violet 450
Applications:	FC
Reactivity:	Mouse
Isotype:	Rat IgG2a, kappa
Research Interest:	Innate Immunity
Cell Type:	Monocytes and Macrophages, NK and NKT Cells, T Cells
Preparation:	The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Formulation:	Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
References:	Fleming, T. J., Fleming, M. L., & Malek, T. R. (1993). Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family.The Journal of Immunology, 151(5), 2399-2408. Daley, J. M., Thomay, A. A., Connolly, M. D., Reichner, J. S., & Albina, J. E. (2008). Use of Ly6G-specific monoclonal antibody to deplete neutrophils in mice. Journal of leukocyte biology, 83(1), 64-70. Dietlin, T. A., Hofman, F. M., Lund, B. T., Gilmore, W., Stohlman, S. A., & van der Veen, R. C. (2007). Mycobacteria-induced Gr-1+ subsets from distinct myeloid lineages have opposite effects on T cell expansion. Journal of leukocyte biology, 81(5), 1205-1212.

Availability: In stock

Size	Price	Qty
25 μg	$85.00
100 μg	$205.00

Additional 1A8 Formats

Cat.No.	Format	Starting From
83112-87	APC-Cyanine7	$ 80.00
83112-50	FITC	$ 60.00
83112-80	APC	$ 70.00
83112-70	PerCP-Cyanine5.5	$ 85.00
83112-40	BG Violet 450	$ 85.00
83112-25	SAFIRE Purified	$ 135.00
83112-60	PE	$ 50.00
83112-77	PE-Cyanine7	$ 80.00
83112-45	BG Violet 500	$ 85.00