Anti-Human CD8a BG Violet 450
The OKT8 monoclonal antibody specifically reacts with human CD8α molecule, a tyPEI transmembrane glycoprotein of 32-34 kDa. CD8α is a member of the Ig superfamily, expressed as a homodimer (CD8αα) or as a heterodimer (CD8αβ). CD8+ αβ T lymphocytes express both CD8αα and CD8αβ, while some T lymphocytes and the natural killer cells express only the homodimers. CD8 binds to MHC class I and influences the development and the activation of T lymphocytes.
OKT8, RPA-T8, and HIT8a antibodies do not compete with each other for binding to peripheral leukocytes, meaning that that they do not recognize the same epitoPEor block each other by steric hindrance.
BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.
BG Violet 450
|Research Interest:||Adaptive Immunity|
|Cell Type:||NK and NKT Cells, T Cells, Dendritic Cells|
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Sayos, J., Wu, C., Morra, M., Wang, N., Zhang, X., Allen, D., ... & Terhorst, C. (1998). The X-linked lymphoproliferative-disease gene product SAP regulates signals induced through the co-receptor SLAM. Nature, 395(6701), 462-469.
Thomas, Y., Sosman, J., Irigoyen, O., Friedman, S. M., Kung, P. C., Goldstein, G., & Chess, L. (1980). Functional analysis of human T cell subsets defined by monoclonal antibodies. I. Collaborative TT interactions in the immunoregulation of B cell differentiation. The Journal of Immunology, 125(6), 2402-2408.
Campanelli, R., Palermo, B., Garbelli, S., Mantovani, S., Lucchi, P., Necker, A., ... & Giachino, C. (2002). Human CD8 co–receptor is strictly involved in MHC–peptide tetramer–TCR binding and T cell activation. International immunology, 14(1), 39-44.