Anti-Mouse CD8a SAFIRE Purified

Grouped product items
SizePrice Qty
100 μg
500 μg
1 mg

Additional 53-6.7 Formats

Cat.No.FormatStarting From
10122-60PE$ 30.00
10122-65PE-Cyanine5$ 65.00
10122-77PE-Cyanine7$ 60.00
10122-70PerCP-Cyanine5.5 $ 70.00
10122-20Purified$ 80.00
10122-25SAFIRE Purified$ 105.00
10122-40BG Violet 450$ 90.00
10122-50FITC$ 25.00
10122-30Biotin$ 35.00
10122-87APC-Cyanine7 $ 75.00
10122-80APC$ 50.00
Catalog Number : 10122-25


The 53-6.7 monoclonal antibody specifically reacts with Ly-2, the 38 kDa alpha chain, and with Lyt-2, the 34 kDa alpha’ chain, of the mouse CD8 antigen. The alpha’ chain is the truncated form of alpha chain, encoded by the same CD8a gene. In CD8a, the alpha and alpha’ chains form heterodimers with CD8b (the beta chains) or homodimers (alpha-alpha), which occur as receptors on the surface of the majority of thymocytes. A subpopulation of mature T lymphocytes expresses the CD8 alpha beta (alpha beta TCR T cells), and a subpopulation of intestinal intraepithelial lymphocytes and dendritic cells express CD8a without CD8b.CD8 interacts with the mouse major histocompatibility complex class I (MHC class I) molecules on antigen-presenting cells or epithelial cells. Its function seems to be to attenuate the CD8-mediated signal for the stimulation of intrathymic T-cell maturation.

The 53-6.7 antibody is useful for depleting CD8+ peripheral T lymphocytes. It cross reacts with the alpha- and alpha’-like polypeptides on some thymic and peripheral lymphocytes.

Additional Information

SAFIRE Purified
Rat IgG2a, kappa
Research Interest:Adaptive Immunity
Cell Type:NK and NKT Cells, T Cells
Application:FC, FA, IHC, IP
The product should be stored undiluted at 4°C. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.
Phosphate-buffered aqueous solution, ph7.2.

Ledbetter, J. A., & Herzenberg, L. A. (1979). Xenogeneic Monoclonal Antibodies to Mouse Lymphoid Differentiation Antigens*. Immunological reviews47(1), 63-90.

Ledbetter, J. A., Rouse, R. V., Micklem, H. S., & Herzenberg, L. A. (1980). T cell subsets defined by expression of Lyt-1, 2, 3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. The Journal of experimental medicine,152(2), 280-295.

Hathcock, K. (1991). T cell enrichment by cytotoxic elimination of B cells and accessory cells. Current protocols in immunology, 3-3.

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