Anti-Mouse CD45.2 BG Violet 500

Grouped product items
Size Price Qty
25 ug
$85.00
100 ug
$210.00

Additional 104 Formats

Cat.No. Format Starting From
07532-60 PE $ 60.00
07532-70 PerCP-Cyanine5.5 $ 65.00
07532-40 BG Violet 450 $ 75.00
07532-20 Purified $ 80.00
07532-50 FITC $ 40.00
07532-87 APC-Cyanine7 $ 95.00
07532-80 APC $ 55.00
07532-77 PE-Cyanine7 $ 55.00
07532-45 BG Violet 500 $ 85.00
Catalog Number : 07532-45

description

The 104 monoclonal antibody specifically reacts with the mouse CD45 molecule, the Leukocyte Common Antigen (LCA) which occurs in the alloantigen CD45.2-expressing mouse strains (C57BL/6, CBA, 129, A, AKR, C58, DBA/1, DBA/2, BALB/c, and C3H/He). The 104 monoclonal antibody does not react with the leukocytes of the CD45.1-expressing mouse strains (DA, SJL/J, RIII, and STS/A).

The CD45 molecule is a member of the Protein Tyrosine Phosphatase (PTP) family, because its intracellular region contains two PTP domains. The extracellular region’s variability is caused by different levels of glycosylation, and the splicing of the 4, 5, and 6 exons.

The isoforms found in the mouse strains depend on the activation state, maturation stage and cell type, and are very important in B and T lymphocytes antigen receptor signal transduction. The 104 antibody inhibits some of the B lymphocytes responses, reduces the serum IgG levels, and influences the autoimmune renal pathology.

BG Violet 500 conjugate is an alternative to the Pacific Orange and BD Horizon V500 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 500nm.

Additional Information

Clone:
104
Format:
BG Violet 500
Applications:
FC
Reactivity:
Mouse
Isotype:
Mouse IgG2a, kappa
Research Interest: Adaptive Immunity
Cell Type: T Cells, NK and NKT Cells, Macrophage / Monocyte, B Cells
Application: FC
Clone: 104
Preparation:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Formulation:
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
References:

Shen, F. W., Tung, J. S., & Boyse, E. A. (1986). Further definition of the Ly-5 system. Immunogenetics24(3), 146-149.

Shen, F.W. (1981) Monoclonal antibodies to mouse lymphocyte differentiation alloantigens. Monoclonal Antibodies and T-Cell Hybridomas: Perspectives and Technical Advances. G.J. Hämmerling, U. Hämmerling, and J.F. Kearney, editors. Elsevier/North-Holland, Amsterdam. pp. 25–31.

Yakura, H., Shen, F. W., Bourcet, E., & Boyse, E. A. (1983). On the function of Ly-5 in the regulation of antigen-driven B cell differentiation. Comparison and contrast with Lyb-2. The Journal of experimental medicine157(4), 1077-1088.

Suzuki, K., Oida, T., Hamada, H., Hitotsumatsu, O., Watanabe, M., Hibi, T., ... & Ishikawa, H. (2000). Gut cryptopatches: direct evidence of extrathymic anatomical sites for intestinal T lymphopoiesis. Immunity13(5), 691-702.

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