Anti-Human/Mouse CD45R (B220) BG Violet 500

Grouped product items
Size Price Qty
25 ug
100 ug

Additional RA3-6B2 Formats

Cat.No. Format Starting From
07131-60 PE $ 40.00
07131-65 PE-Cyanine5 $ 65.00
07131-70 PerCP-Cyanine5.5 $ 70.00
07131-77 PE-Cyanine7 $ 70.00
07131-40 BG Violet 450 $ 90.00
07131-20 Purified $ 70.00
07131-50 FITC $ 30.00
07131-30 Biotin $ 60.00
07131-80 APC $ 50.00
07131-45 BG Violet 500 $ 85.00
Catalog Number : 07131-45


The RA3-6B2 monoclonal antibody specifically reacts with a 220 kDa exon A-restricted epitope on the extracellular domain of mouse CD45 glycoprotein. CD45R/B220 is expressed on all the development stages of B cells, and at a low level on plasma cells and a subset of memory B cells. Low levels of CD45R/B220 are also expressed by some activated T lymphocytes, natural killer cell progenitors in the bone marrow, lymphokine activated killer cells (LAK), T lymphocytes of lpr/lpr mutant mouse, apoptotic T cells of mice injected with bacterial superantigens, and macrophage progenitors in fetal liver.

The CD45 molecule is a member of the Protein Tyrosine Phosphatase (PTP) family, because its intracellular region contains two PTP domains. The extracellular region’s variability is caused by different levels of glycosylation, and the splicing of the 4, 5, and 6 exons. The isoforms found in the mouse strains depend on the activation state, maturation stage and cell type, and are very important in B and T lymphocytes antigen receptor signal transduction.

RPA-T8, OKT8, and HIT8a antibodies do not compete with each other for binding to peripheral leukocytes, meaning that that they do not recognize the same epitope or block each other by steric hindrance.

BG Violet 500 conjugate is an alternative to the Pacific Orange and BD Horizon V500 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 500nm.

Additional Information

BG Violet 500
Human, Mouse
Rat IgG2a, kappa
Research Interest: Adaptive Immunity
Cell Type: T Cells, B Cells
Application: FC
Clone: RA3-6B2
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Coffman, R. L. (1983). Surface Antigen Expression and Immunoglobulin Gene Rearrangement During Mouse pre-B Cell Development. Immunological reviews,69(1), 5-23.

Allman, D. M., Ferguson, S. E., & Cancro, M. P. (1992). Peripheral B cell maturation. I. Immature peripheral B cells in adults are heat-stable antigenhi and exhibit unique signaling characteristics. The Journal of Immunology,149(8), 2533-2540.

Monteith, C. E., Chelack, B. J., Davis, W. C., & Haines, D. M. (1996). Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues.Canadian journal of veterinary research60(3), 193.

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