Anti-MouseCD62L (L-Selectin) PE-Cyanine7
The MEL-14 monoclonal antibody specifically reacts with L- selectin (CD62L), a receptor with lectin-like and Epidermal Growth Factor-like domains. The weight of the CD62L molecules depend on their origin: 74 kDa (on lymphocytes) or 95 kDa (on neutrophils).
In the Mouseorganism, CD62L can be expressed by most thymocytes, on subsets of B and T lymphocytes, monocytes, eosinophils, and neutrophils. The L-selectin binds sulfated, fucosylated, and glycosylated glycoproteins (MadCAM-1, GLYCAM-1, and CD 34). It mediates the migration of lymphocytes to the site of inflammation and their return to the peripheral lymphoid tissues and to the HEV (high endothelial venules). In vitro, L-selectin inhibits this binding and the lymphocyte extravasation into peripheral lymph nodes.
Rat IgG2a, kappa
|Research Interest:||Adaptive Immunity, Angiogenesis|
|Cell Type:||B Cells, Macrophage / Monocyte, NK and NKT Cells, T Cells|
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Gallatin, W. M., Weissman, I. L., & Butcher, E. C. (1982). A cell-surface molecule involved in organ-specific homing of lymphocytes. Nature, 304(5921), 30-34.
Siegelman, M. H., Cheng, I. C., Weissman, I. L., & Wakeland, E. K. (1990). The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding. Cell, 61(4), 611-622.
Pizcueta, P., & Luscinskas, F. W. (1994). Monoclonal antibody blockade of L-selectin inhibits mononuclear leukocyte recruitment to inflammatory sites in vivo. The American journal of pathology, 145(2), 461.