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Anti-Mouse CD8a BG Violet 450



Catalog Number : 10122-40

Description

The 53-6.7 monoclonal antibody specifically reacts with Ly-2, the 38 kDa alpha chain, and with Lyt-2, the 34 kDa alpha’ chain, of the mouse CD8 antigen. The alpha’ chain is the truncated form of alpha chain, encoded by the same CD8a gene. In CD8a, the alpha and alpha’ chains form heterodimers with CD8b (the beta chains) or homodimers (alpha-alpha), which occur as receptors on the surface of the majority of thymocytes. A subpopulation of mature T lymphocytes expresses the CD8 alpha beta (alpha beta TCR T cells), and a subpopulation of intestinal intraepithelial lymphocytes and dendritic cells express CD8a without CD8b.CD8 interacts with the mouse major histocompatibility complex class I (MHC class I) molecules on antigen-presenting cells or epithelial cells. Its function seems to be to attenuate the CD8-mediated signal for the stimulation of intrathymic T-cell maturation.

The 53-6.7 antibody is useful for depleting CD8+ peripheral T lymphocytes. It cross reacts with the alpha- and alpha’-like polypeptides on some thymic and peripheral lymphocytes.

BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.

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C57Bl/6 splenocytes were stained with BG Violet 450 53-6.7 with relevant isotype control in Red.
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Additional Information

Clone
53-6.7
Format:
BG Violet 450
Applications
FC
Reactivity
Mouse
Isotype:
Rat IgG2a, kappa
Research Interest: Adaptive Immunity
Cell Type: NK and NKT Cells, T Cells
Preperation:
The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Formulation:
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
References:

Ledbetter, J. A., & Herzenberg, L. A. (1979). Xenogeneic Monoclonal Antibodies to Mouse Lymphoid Differentiation Antigens*. Immunological reviews47(1), 63-90.

Ledbetter, J. A., Rouse, R. V., Micklem, H. S., & Herzenberg, L. A. (1980). T cell subsets defined by expression of Lyt-1, 2, 3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. The Journal of experimental medicine,152(2), 280-295.

Hathcock, K. (1991). T cell enrichment by cytotoxic elimination of B cells and accessory cells. Current protocols in immunology, 3-3.


Availability: In stock

Size Price Qty
25 μg
$90.00
100 μg
$190.00
Additional 53-6.7 Formats
Cat.No. Format Starting From
10122-60PE $ 30.00
10122-65PE-Cyanine5 $ 65.00
10122-77PE-Cyanine7 $ 60.00
10122-70PerCP-Cyanine5.5 $ 70.00
10122-20Purified $ 80.00
10122-25SAFIRE Purified $ 105.00
10122-40BG Violet 450 $ 90.00
10122-50FITC $ 25.00
10122-30Biotin $ 35.00
10122-87APC-Cyanine7 $ 75.00
10122-80APC $ 50.00