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Anti-Mouse CD357 (GITR) SAFIRE Purified



Catalog Number : 39612-25

Description

The DTA-1 monoclonal antibody specifically reacts with MouseGlucocorticoid-Induced TNFR-related protein, also known as GITR and TNFRSF18, a 66-70 kDa homodimer glycoprotein, detected in the T cells treated with glucocorticoid dexamethasone. GITR is also expressed in naïve mice by CD25+/CD4+/CD8a- thymocytes and on CD25+/CD4+/CD45RB-low splenocytes. Low levels were detected in splenic CD25+/CD4+/CD45RB-low T cells, B cells, dendritic cells and macrophages. A GITR ligand was detected on dendritic cells, macrophages and B cells.The DTA-1 antibody stimulates GITR and abrogates suppression by T regulatory cells (Treg), without affecting their proliferation.

DTA-1 administration or the removal of GITR-expressing cells led to organ specific autoimmune disease.

Additional Information

Clone
DTA-1
Format:
SAFIRE Purified
Applications
FC, FA, IHC/IF
Reactivity
Mouse
Isotype:
Rat IgG2b
Research Interest: Adaptive Immunity
Cell Type: T Cells, Treg cells
Preperation:
The product should be stored undiluted at 4°C. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.
Formulation:
Phosphate-buffered aqueous solution, ph7.2.
References:

Ko, K., Yamazaki, S., Nakamura, K., Nishioka, T., Hirota, K., Yamaguchi, T., ... & Sakaguchi, S. (2005). Treatment of advanced tumors with agonistic anti-GITR mAb and its effects on tumor-infiltrating Foxp3+ CD25+ CD4+ regulatory T cells. The Journal of experimental medicine202(7), 885-891.

Shimizu, J., Yamazaki, S., Takahashi, T., Ishida, Y., & Sakaguchi, S. (2002). Stimulation of CD25+ CD4+ regulatory T cells through GITR breaks immunological self-tolerance. Nature immunology3(2), 135-142.

Tone, M., Tone, Y., Adams, E., Yates, S. F., Frewin, M. R., Cobbold, S. P., & Waldmann, H. (2003). Mouse glucocorticoid-induced tumor necrosis factor receptor ligand is costimulatory for T cells. Proceedings of the National Academy of Sciences100(25), 15059-15064.


Availability: In stock

Size Price Qty
100 μg
$250.00
500 μg
$500.00
Additional DTA-1 Formats
Cat.No. Format Starting From
39612-60PE $ 90.00
39612-50FITC $ 100.00
39612-80APC $ 115.00
39612-25SAFIRE Purified $ 250.00