Anti-Mouse CD357 (GITR) SAFIRE Purified
description
The DTA-1 monoclonal antibody specifically reacts with MouseGlucocorticoid-Induced TNFR-related protein, also known as GITR and TNFRSF18, a 66-70 kDa homodimer glycoprotein, detected in the T cells treated with glucocorticoid dexamethasone. GITR is also expressed in naïve mice by CD25+/CD4+/CD8a- thymocytes and on CD25+/CD4+/CD45RB-low splenocytes. Low levels were detected in splenic CD25+/CD4+/CD45RB-low T cells, B cells, dendritic cells and macrophages. A GITR ligand was detected on dendritic cells, macrophages and B cells.The DTA-1 antibody stimulates GITR and abrogates suppression by T regulatory cells (Treg), without affecting their proliferation.
DTA-1 administration or the removal of GITR-expressing cells led to organ specific autoimmune disease.
Additional Information
Clone:
|
DTA-1
|
Format:
|
SAFIRE Purified
|
Applications:
|
FC, FA, IHC/IF
|
Reactivity:
|
Mouse
|
Isotype:
|
Rat IgG2b
|
Research Interest: | Adaptive Immunity |
---|---|
Cell Type: | T Cells, T Regulatory Cells |
Application: | FC, FA, IHC/IF |
Clone: | DTA-1 |
Preparation:
|
The product should be stored undiluted at 4°C. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.
|
Formulation:
|
Phosphate-buffered aqueous solution, ph7.2.
|
References:
|
Ko, K., Yamazaki, S., Nakamura, K., Nishioka, T., Hirota, K., Yamaguchi, T., ... & Sakaguchi, S. (2005). Treatment of advanced tumors with agonistic anti-GITR mAb and its effects on tumor-infiltrating Foxp3+ CD25+ CD4+ regulatory T cells. The Journal of experimental medicine, 202(7), 885-891. Shimizu, J., Yamazaki, S., Takahashi, T., Ishida, Y., & Sakaguchi, S. (2002). Stimulation of CD25+ CD4+ regulatory T cells through GITR breaks immunological self-tolerance. Nature immunology, 3(2), 135-142. Tone, M., Tone, Y., Adams, E., Yates, S. F., Frewin, M. R., Cobbold, S. P., & Waldmann, H. (2003). Mouse glucocorticoid-induced tumor necrosis factor receptor ligand is costimulatory for T cells. Proceedings of the National Academy of Sciences, 100(25), 15059-15064. |