Murine LIX (CXCL6) Pre-Coated ELISA Kit
description
The BioGems Murine LIX (CXCL6) Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure LIX (CXCL6) with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for LIX (CXCL6). It contains E. coli-expressed recombinant LIX (CXCL6). The detection antibody is a biotinylated goat polyclonal antibody specific for LIX (CXCL6). The kit is analytically validated with ready to use reagents.
Product Details
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Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<10pg/ml
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Range:
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15.6pg/ml-1000pg/ml
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Specificity:
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Natural and recombinant Murine LIX (CXCL6)
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Uniprot:
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P50228
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 - 8 °C.
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Components
Description | Quantity | Volume |
Anti-Murine LIX (CXCL6) Pre-coated 96-well strip microplate | 1 | 12 strips of 8 wells |
Murine LIX (CXCL6) Standard | 2 | 10 ng vial |
Murine LIX (CXCL6) Biotinylated antibody (100x) | 1 | 130 µL |
Avidin-Biotin-Peroxidase Complex (100x) | 1 | 130 µL |
Sample Diluent | 1 | 30mL |
Antibody Diluent | 1 | 12mL |
Avidin-Biotin-Peroxidase Diluent | 1 | 12mL |
Wash Buffer Concentrate (Powder for 1000mL) | 1 | Pack |
Color Developing Reagent | 1 | 10mL |
Stop Solution | 1 | 10mL |
Plate Sealers | 4 | Each |
Reproducibility
| Intra-Assay | Inter-Assay | ||||
Sample ID | 1 | 2 | 3 | 1 | 2 | 3 |
n= | 16 | 16 | 16 | 24 | 24 | 24 |
Mean Measured Concentration (pg/mL) | 89 | 238 | 496 | 103 | 276 | 513 |
Standard Deviation | 4.54 | 13.3 | 18.9 | 7.42 | 20.7 | 32.8 |
Consistency (%CV) | 5.1 | 5.6 | 3.8 | 7.2 | 7.5 | 6.4 |