Murine LIX (CXCL6) Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$425.00
Catalog Number : BGK50228

description

CXCL6, also known as LIX in mice, is a CXC chemokine that signals through the CXCR2 receptor. It is expressed in monocytes, platelets, endothelial cells, and mast cells. LIX is a chemoattractant for neutrophils. The two naturally occurring variants of LIX, LIX 1-78 (GCP-2) and LIX 9-78 (GCP-2), contain 78 and 70 amino acid residues, respectively. LIX contains the four conserved cysteine residues present in CXC chemokines, and also contains the ‘ELR’ motif common to CXC chemokines that binds to the CXCR1 and CXCR2 receptors.

The BioGems Murine LIX (CXCL6) Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure LIX (CXCL6) with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for LIX (CXCL6). It contains E. coli-expressed recombinant LIX (CXCL6). The detection antibody is a biotinylated goat polyclonal antibody specific for LIX (CXCL6). The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<10pg/ml
Range:
15.6pg/ml-1000pg/ml
Specificity:
Natural and recombinant Murine LIX (CXCL6)
Uniprot:
P50228
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Murine LIX (CXCL6) Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Murine LIX (CXCL6) Standard

2

10 ng vial

Murine LIX (CXCL6) Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

89

238

496

103

276

513

Standard Deviation

4.54

13.3

18.9

7.42

20.7

32.8

Consistency (%CV)

5.1

5.6

3.8

7.2

7.5

6.4

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