Murine LIF Pre-Coated ELISA Kit

LIF is a pleiotrophic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor.

The BioGems Murine LIF Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure LIF with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for LIF. It contains E. coli-expressed recombinant LIF. The detection antibody is a biotinylated goat polyclonal antibody specific for LIF. The kit is analytically validated with ready to use reagents.