Human TNF-α Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$345.00
Catalog Number : BGK01375

description

TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells, including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands, and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells, and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, and diabetes. Human and murine TNF-α demonstrate significant cross-species reactivity. TNF-α exists in two forms; a type II transmembrane protein, and a mature soluble protein. The TNF-α transmembrane protein is proteolitically cleaved to yield a soluble, biologically active, 17 kDa TNF-α, which forms a non-covalently linked homotrimer in solution.

The BioGems Human TNF-α Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human TNF-α with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for TNF-α. It contains E. coli-expressed recombinant Human TNF-α. The detection antibody is a biotinylated goat polyclonal antibody specific for TNF-α. The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<1 pg/ml
Range:
15.6 pg/mL- 1,000 pg/mL
Specificity:
Natural and recombinant Human TNF-α
Uniprot:
P01375
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human TNF-α Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human TNF-α Standard

2

10 ng vial

Human TNF-α Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

93

327

608

102

319

613

Standard Deviation

5.1

15.4

31

7.65

15.3

35

Consistency (%CV)

5.5

4.7

5.1

7.5

4.8

5.7

© BioGems International, Inc. All rights reserved.