Human MIF Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$465.00
Catalog Number : BGK4AY87

description

Macrophage migration inhibitory factor (MIF) is a small secreted protein that can act as a pleiotropic pro-inflammatory cytokine, as well as an enzyme. MIF pro-inflammatory activity can be initiated by signaling through CD74 and CD44, resulting in the secretion of TNF-a, IL-1, IL-6, IL-8, and various MMPs. The enzymatic activity of MIF is characterized by its ability to act as a tautomerase, capable of catalyzing the keto-to-enol isomerization of keto-phenylpyruvate and L-dopachrome. It appears as though MIF catalytic activity is dependent upon a trimeric configuration and a free N-terminal proline residue.

The BioGems Human MIF Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure MIF with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for MIF. It contains E. coli-expressed recombinant MIF. The detection antibody is a biotinylated goat polyclonal antibody specific for MIF. The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<20pg/ml
Range:
156pg/ml-10,000pg/ml
Specificity:
Natural and recombinant Human MIF
Uniprot:
I4AY87
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human MIF Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human MIF Standard

2

10 ng vial

Human MIF Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

1442

3826

6427

1625

4177

6782

Standard Deviation

90.85

206.6

315

121.9

254.8

393.4

Consistency (%CV)

6.3

5.4

4.9

7.5

6.1

5.8

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