Human MCP-4 (CCL13) Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$440.00
Catalog Number : BGK99616

description

The MCP proteins are members of the CC chemokine family that signal through CCR2 and, with the exception of MCP-1, other CCR receptors. The MCP proteins chemoattract and activate monocytes, activated Tcells, basophils, NK cells, and immature dendritic cells. The MCP family cross-reacts across species.

The BioGems Human MCP-4 (CCL13) Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure MCP-4 (CCL13) with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for MCP-4 (CCL13). It contains E. coli-expressed recombinant MCP-4 (CCL13). The detection antibody is a biotinylated goat polyclonal antibody specific for MCP-4 (CCL13). The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<10pg/ml
Range:
7.8pg/ml-500pg/ml
Specificity:
Natural and recombinant Human MCP-4 (CCL13) Pre-Coated ELISA Kit
Uniprot:
Q99616
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human MCP-4 (CCL13) Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human MCP-4 (CCL13) Standard

2

10 ng vial

Human MCP-4 (CCL13) Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

50

191

325

54

223

356

Standard Deviation

2.15

8.98

20.15

3.13

14.05

27.77

Consistency (%CV)

4.3

4.7

6.2

5.8

6.3

7.8

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