Human IL-6 Receptor Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$460.00
Catalog Number : BGK0N0L5

description

IL-6 mediates its biological effects through the type I IL-6 receptor system that consists of two chains, IL-6Rα and gp130. While the IL-6Rα chain is the binding component specific to IL-6, the gp130 chain only transmits signals of IL-6 when bound to IL-6Rα. The gp130 can also transmit signals from LIF, OSM, CNTF, IL-11 and CT-1 in conjunction with other receptor subunits. The low-affinity binding site for IL-6 is composed of IL-6Rα alone. IL-6Rα is expressed in a wide range of cells, including T cells, fibroblasts and macrophages.

The BioGems Human IL-6 Receptor Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure IL-6 Receptor with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-6 Receptor. It contains sf21-expressed recombinant IL-6 Receptor. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-6 Receptor. The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<10pg/ml
Range:
31.2pg/ml-2000pg/ml
Specificity:
Natural and recombinant Human IL-6 Receptor Pre-Coated ELISA Kit
Uniprot:
A0N0L5
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human IL-6 Receptor Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human IL-6 Receptor Standard

2

10 ng vial

Human IL-6 Receptor Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

115

768

1558

124

845

1334

Standard Deviation

9.2

26.11

60.76

10.54

47.32

81.37

Consistency (%CV)

8

3.4

3.9

8.5

5.6

6.1

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