Human IL-6 Pre-Coated ELISA Kit
description
IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6R α and gp130. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells.
The BioGems Human IL-6 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure human IL-6 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-6. It contains E. coli-expressed recombinant human IL-6. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-6. The kit is analytically validated with ready to use reagents.
Product Details
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Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<0.3pg/ml
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Range:
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4.69 pg/mL – 300 pg/mL
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Specificity:
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Natural and recombinant Human IL-6
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Uniprot:
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P05231
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 - 8 °C.
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Components
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Description |
Quantity |
Volume |
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Anti-Human IL-6 Pre-coated 96-well strip microplate |
1 |
12 strips of 8 wells |
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Human IL-6 Standard |
2 |
10 ng vial |
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Human IL-6 Biotinylated Conjugate (100x) |
1 |
130 µL |
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Avidin-Biotin-Peroxidase Complex (100x) |
1 |
130 µL |
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Sample Diluent |
1 |
30mL |
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Antibody Diluent |
1 |
12mL |
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Avidin-Biotin-Peroxidase Diluent |
1 |
12mL |
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Wash Buffer Concentrate (Powder for 1000mL) |
1 |
Pack |
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Color Developing Reagent |
1 |
10mL |
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Stop Solution |
1 |
10mL |
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Plate Sealers |
4 |
Each |
Reproducibility
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Intra-Assay |
Inter-Assay |
||||
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Sample ID |
1 |
2 |
3 |
1 |
2 |
3 |
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n= |
16 |
16 |
16 |
24 |
24 |
24 |
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Mean Measured Concentration (pg/mL) |
16.3 |
98 |
179 |
18.2 |
99 |
185 |
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Standard Deviation |
0.8 |
2.3 |
4.2 |
1 |
3.6 |
5.7 |
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Consistency (%CV) |
4.9 |
2.3 |
2.3 |
5.5 |
3.6 |
3.1 |