Human IL-6 Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$345.00
Catalog Number : BGK05231

description

IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6R α and gp130. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells.

The BioGems Human IL-6 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure human IL-6 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-6. It contains E. coli-expressed recombinant human IL-6. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-6. The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<0.3pg/ml
Range:
4.69 pg/mL – 300 pg/mL
Specificity:
Natural and recombinant Human IL-6
Uniprot:
P05231
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human IL-6 Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human IL-6 Standard

2

10 ng vial

Human IL-6 Biotinylated Conjugate (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

16.3

98

179

18.2

99

185

Standard Deviation

0.8

2.3

4.2

1

3.6

5.7

Consistency (%CV)

4.9

2.3

2.3

5.5

3.6

3.1

 

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