Human IL-2 Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$345.00
Catalog Number : BGK60568

description

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes.

The BioGems Human IL-2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human IL-2 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-2. It contains E. coli-expressed recombinant Human IL-2. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-2. The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<0.1 pg/ml
Range:
15.6 pg/mL- 1,000 pg/mL
Specificity:
Natural and recombinant Human IL-2
Uniprot:
P60568
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human IL-2 Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human IL-2 Standard

2

10 ng vial

Human IL-2 Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

75

272

537

96

310

643

Standard Deviation

3.45

9.52

16.65

6.048

16.12

39.2

Consistency (%CV)

4.6

3.5

3.1

6.3

5.2

6.1

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