Human IL-10 Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$345.00
Catalog Number : BGK22301

description

IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes.  IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF-α.  Like IL-10, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions.  Human IL-10 is active on murine cells, but murine IL-10 is inactive on human cells.

The BioGems Human IL-10 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human IL-10 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-10. It contains E. coli-expressed recombinant Human IL-10. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-10. The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<0.5 pg/ml
Range:
7.8 pg/mL- 500 pg/mL
Specificity:
Natural and recombinant Human IL-10
Uniprot:
P22301
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human IL-10 Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human IL-10 Standard

2

10 ng vial

Human IL-10 Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

25

112

280

34

184

312

Standard Deviation

1.35

5.38

8.68

2.55

14.54

20.9

Consistency (%CV)

5.4

4.8

3.1

7.5

7.9

6.7

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