Human IL-1β Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$345.00
Catalog Number : BGK01584

description

IL-1β is a proinflammatory cytokine produced in a variety of cells, including monocytes, tissue macrophages, keratinocytes, and other epithelial cells.  Both IL-1α and IL-1β bind to the same receptor and have similar, if not identical, biological properties.  These cytokines have a broad range of activities including the stimulation of thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity, and the release of prostaglandin and collagenase from synovial cells.  However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine.

The BioGems Human IL-1β Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure IL-1&beta with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-1&beta. It contains E. coli-expressed recombinant Human IL-1&beta. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-1&beta. The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<.15 pg/ml
Range:
3.9 pg/mL- 250 pg/mL
Specificity:
Natural and recombinant Human IL-1β
Uniprot:
P01584
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human IL-1β Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human IL-1β Standard

2

1 ng vial

Human IL-1β Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

19

41.2

116

27.5

61.3

179.1

Standard Deviation

1

2.6

6.5

1.6

4.1

12.7

Consistency (%CV)

5.3

6.3

5.6

5.8

6.7

7.1

© BioGems International, Inc. All rights reserved.