Human FLT-3 Ligand Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$405.00
Catalog Number : BGK49771

description

Flt3-Ligand is a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand binds to cells expressing the tyrosine kinase receptor Flt3. Flt3-Ligand, by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation. Unlike SCF, Flt3-Ligand exerts no activity on mast cells. Multiple isoforms of Flt3-Ligand have been identified. The predominant biologically active form is anchored to the cell surface as the extracellular domain of a transmembrane protein (209 a.a.). The membrane-bound isoform can be proteolytically cleaved to generate a biologically active soluble isoform.

The BioGems Human FLT-3 Ligand Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure FLT-3 Ligand with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for FLT-3 Ligand. It contains sf21-expressed recombinant FLT-3 Ligand. The detection antibody is a biotinylated goat polyclonal antibody specific for FLT-3 Ligand. The kit is analytically validated with ready to use reagents.

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Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<10pg/ml
Range:
15.6pg/ml-1000pg/ml
Specificity:
Natural and recombinant Human FLT-3 Ligand
Uniprot:
P49771
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human FLT-3 Ligand Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human FLT-3 Ligand Standard

2

10 ng vial

Human FLT-3 Ligand Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

109

256

520

112

245

518

Standard Deviation

3.49

8.45

22.88

10.64

18.38

41.96

Consistency (%CV)

3.2

3.3

4.4

9.5

7.5

8.1

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