Human FGF-basic Pre-Coated ELISA Kit
description
The BioGems Human FGF-basic Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure TLR-3 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for FGF-basic . It contains E. coli-expressed recombinant FGF-basic. The detection antibody is a biotinylated goat polyclonal antibody specific for FGF-basic. The kit is analytically validated with ready to use reagents.
Product Details
|
Assay Type:
|
Solid Phase Sandwich ELISA: 96-Well Strip Plate
|
|
Sensitivity:
|
<10pg/ml
|
|
Range:
|
62.5pg/ml-4,000pg/ml
|
|
Specificity:
|
Natural and recombinant Human FGF-basic
|
|
Uniprot:
|
P09038
|
|
Interference:
|
No detectable cross-reactivity with other relevant proteins
|
|
Storage Conditions:
|
Store the unopened product at 2 - 8 °C.
|
Components
Description | Quantity | Volume |
Anti-Human FGF-basic Pre-coated 96-well strip microplate | 1 | 12 strips of 8 wells |
Human FGF-basic Standard | 2 | 10 ng vial |
Human FGF-basic Biotinylated antibody (100x) | 1 | 130 µL |
Avidin-Biotin-Peroxidase Complex (100x) | 1 | 130 µL |
Sample Diluent | 1 | 30mL |
Antibody Diluent | 1 | 12mL |
Avidin-Biotin-Peroxidase Diluent | 1 | 12mL |
Wash Buffer Concentrate (Powder for 1000mL) | 1 | Pack |
Color Developing Reagent | 1 | 10mL |
Stop Solution | 1 | 10mL |
Plate Sealers | 4 | Each |
Reproducibility
|
|
Intra-Assay |
Inter-Assay |
||||
|
Sample ID |
1 |
2 |
3 |
1 |
2 |
3 |
|
n= |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean Measured Concentration (pg/mL) |
150 |
752 |
1076 |
135 |
781 |
1071 |
|
Standard Deviation |
10.95 |
54.89 |
79.62 |
10.8 |
62.48 |
92.1 |
|
Consistency (%CV) |
7.3 |
7.3 |
7.4 |
8 |
8 |
8.6 |