Human FGF-basic Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$460.00
Catalog Number : BGK09038

description

FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated, heparin-binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland, liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4.

The BioGems Human FGF-basic Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure TLR-3 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for FGF-basic . It contains E. coli-expressed recombinant FGF-basic. The detection antibody is a biotinylated goat polyclonal antibody specific for FGF-basic. The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<10pg/ml
Range:
62.5pg/ml-4,000pg/ml
Specificity:
Natural and recombinant Human FGF-basic
Uniprot:
P09038
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human FGF-basic Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human FGF-basic Standard

2

10 ng vial

Human FGF-basic Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

150

752

1076

135

781

1071

Standard Deviation

10.95

54.89

79.62

10.8

62.48

92.1

Consistency (%CV)

7.3

7.3

7.4

8

8

8.6

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