Human BMP-2 Pre-Coated ELISA Kit
description
BMPs (Bone Morphogenetic Proteins) belong to the TGF-β superfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 plays an important role in cardiac morphogenesis, and is expressed in a variety of tissues, including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP-2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. Each BMP-2 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 23 amino acid signal sequence for secretion, and a 259 amino acid propeptide. After dimerization of this precursor, the covalent bonds between the propeptide (which is also a disulfide-linked homodimer) and the mature BMP-2 ligand are cleaved by a furin-type protease.
The BioGems Human BMP-2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure BMP-2 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for BMP-2. It contains CHO-expressed recombinant BMP-2. The detection antibody is a biotinylated goat polyclonal antibody specific for BMP-2. The kit is analytically validated with ready to use reagents.
Product Details
Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<2pg/ml
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Range:
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31.2pg/ml-2000pg/ml
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Specificity:
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Natural and recombinant Human BMP-2
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Uniprot:
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C8C060
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 - 8 °C.
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Components
Description | Quantity | Volume |
Anti-Human BMP-2 Pre-coated 96-well strip microplate | 1 | 12 strips of 8 wells |
Human BMP-2 Standard | 2 | 10 ng vial |
Human BMP-2 Biotinylated antibody (100x) | 1 | 130 µL |
Avidin-Biotin-Peroxidase Complex (100x) | 1 | 130 µL |
Sample Diluent | 1 | 30mL |
Antibody Diluent | 1 | 12mL |
Avidin-Biotin-Peroxidase Diluent | 1 | 12mL |
Wash Buffer Concentrate (Powder for 1000mL) | 1 | Pack |
Color Developing Reagent | 1 | 10mL |
Stop Solution | 1 | 10mL |
Plate Sealers | 4 | Each |
Reproducibility
| Intra-Assay | Inter-Assay | ||||
Sample ID | 1 | 2 | 3 | 1 | 2 | 3 |
n= | 16 | 16 | 16 | 24 | 24 | 24 |
Mean Measured Concentration (pg/mL) | 285 | 742 | 1197 | 306 | 834 | 1358 |
Standard Deviation | 9.12 | 25.97 | 37.12 | 18.97 | 58.38 | 97.78 |
Consistency (%CV) | 3.2 | 3.5 | 3.1 | 6.2 | 7 | 7.2 |