Human April Pre-Coated ELISA Kit

Grouped product items
SizePrice Qty
1 Kit
$410.00
Catalog Number : BGK2QBA2

description

APRIL, a member of the TNF superfamily, is expressed in monocytes, macrophages, certain transformed cell lines, certain cancers of the colon, and lymphoid tissues. APRIL, along with another TNF family member, BAFF, competes for two receptors, TACI and BCMA. APRIL has the ability to stimulate proliferation of various tumor cell lines, including Jurkat T cells and MCF-7 carcinoma cells. Like BAFF, APRIL also stimulates the proliferation of B and T cells. The human APRIL gene codes for at least four alternatively spliced transcriptional variants, which give rise to different isoforms of the APRIL precursor protein. All isoforms can be cleaved by the protease, furin, to release a soluble C-terminal fragment, which comprises the TNF-like receptor binding of the APRIL precursor.

The BioGems Human APRIL Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure APRIL with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for APRIL . It contains NS0-expressed recombinant APRIL . The detection antibody is a biotinylated goat polyclonal antibody specific for APRIL . The kit is analytically validated with ready to use reagents.

product image

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<10pg/ml
Range:
156pg/ml-10,000pg/ml
Specificity:
Natural and recombinant Human APRIL
Uniprot:
Q2QBA2
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human April Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human April Standard

2

10 ng vial

Human April Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

653

2945

5633

864

3214

5366

Standard Deviation

26.8

156.1

219.7

42.4

202.5

268.3

Consistency (%CV)

4.1

5.3

3.9

4.9

6.3

5

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