Murine IL-10 Pre-Coated ELISA Kit
description
IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of pro-inflammatory cytokines such as IL-1 and TNF-α. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 is active on murine cells, but murine IL-10 is inactive on human cells.
The BioGems Murine IL-10 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Murine IL-10 with a 96-well strip plate that is pre-coated with rat monoclonal antibody specific for IL-10. It contains E. coli-expressed recombinant Murine IL-10. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-10. The kit is analytically validated with ready to use reagents.
Product Details
Assay Type:
|
Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<1 pg/ml
|
Range:
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15.6 pg/mL- 1,000 pg/mL
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Specificity:
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Natural and recombinant Murine IL-10
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Uniprot:
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P18893
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 - 8 °C.
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Components
Description |
Quantity |
Volume |
Anti-Murine IL-10 Pre-coated 96-well strip microplate |
1 |
12 strips of 8 wells |
Murine IL-10 Standard |
2 |
10 ng vial |
Murine IL-10 Biotinylated antibody (100x) |
1 |
130 µL |
Avidin-Biotin-Peroxidase Complex (100x) |
1 |
130 µL |
Sample Diluent |
1 |
30mL |
Antibody Diluent |
1 |
12mL |
Avidin-Biotin-Peroxidase Diluent |
1 |
12mL |
Wash Buffer Concentrate (Powder for 1000mL) |
1 |
Pack |
Color Developing Reagent |
1 |
10mL |
Stop Solution |
1 |
10mL |
Plate Sealers |
4 |
Each |
Reproducibility
|
Intra-Assay |
Inter-Assay |
||||
Sample ID |
1 |
2 |
3 |
1 |
2 |
3 |
n= |
16 |
16 |
16 |
24 |
24 |
24 |
Mean Measured Concentration (pg/mL) |
82 |
413 |
724 |
113 |
367 |
628 |
Standard Deviation |
3.77 |
21.5 |
44.2 |
7 |
24.96 |
46.5 |
Consistency (%CV) |
4.6 |
5.2 |
6.1 |
6.2 |
6.8 |
7.4 |