Human TIMP-1 Pre-Coated ELISA Kit
description
The BioGems Human TIMP-1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure TIMP-1 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for TIMP-1. It contains NSO-expressed recombinant TIMP-1. The detection antibody is a biotinylated goat polyclonal antibody specific for TIMP-1. The kit is analytically validated with ready to use reagents.
Product Details
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Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<5pg/ml
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Range:
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156pg/ml-10,000pg/ml
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Specificity:
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Natural and recombinant Human TIMP-1 Pre-Coated ELISA Kit
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Uniprot:
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P01033
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 - 8 °C.
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Components
Description | Quantity | Volume |
Anti-Human TIMP-1 Pre-coated 96-well strip microplate | 1 | 12 strips of 8 wells |
Human TIMP-1 Standard | 2 | 10 ng vial |
Human TIMP-1 Biotinylated antibody (100x) | 1 | 130 µL |
Avidin-Biotin-Peroxidase Complex (100x) | 1 | 130 µL |
Sample Diluent | 1 | 30mL |
Antibody Diluent | 1 | 12mL |
Avidin-Biotin-Peroxidase Diluent | 1 | 12mL |
Wash Buffer Concentrate (Powder for 1000mL) | 1 | Pack |
Color Developing Reagent | 1 | 10mL |
Stop Solution | 1 | 10mL |
Plate Sealers | 4 | Each |
Reproducibility
| Intra-Assay | Inter-Assay | ||||
Sample ID | 1 | 2 | 3 | 1 | 2 | 3 |
n= | 16 | 16 | 16 | 24 | 24 | 24 |
Mean Measured Concentration (pg/mL) | 1.21 | 3.28 | 7.06 | 1.34 | 3.54 | 6.32 |
Standard Deviation | 0.057 | 0.171 | 0.424 | 0.071 | 0.248 | 0.43 |
Consistency (%CV) | 4.7 | 5.2 | 6 | 5.3 | 7 | 6.8 |