Human MIP-3α (CCL20) Pre-Coated ELISA Kit
description
The BioGems Human MIP-3α (CCL20) Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure MIP-3α (CCL20) with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for MIP-3α (CCL20). It contains E. coli-expressed recombinant MIP-3α (CCL20). The detection antibody is a biotinylated goat polyclonal antibody specific for MIP-3α (CCL20). The kit is analytically validated with ready to use reagents.
Product Details
|
Assay Type:
|
Solid Phase Sandwich ELISA: 96-Well Strip Plate
|
|
Sensitivity:
|
<1pg/ml
|
|
Range:
|
7.8pg/ml-500pg/ml
|
|
Specificity:
|
Natural and recombinant Human MIP-3α (CCL20)
|
|
Uniprot:
|
P78556
|
|
Interference:
|
No detectable cross-reactivity with other relevant proteins
|
|
Storage Conditions:
|
Store the unopened product at 2 - 8 °C.
|
Components
Description | Quantity | Volume |
Anti-Human MIP-3α (CCL20) Pre-coated 96-well strip microplate | 1 | 12 strips of 8 wells |
Human MIP-3α (CCL20) Standard | 2 | 10 ng vial |
Human MIP-3α (CCL20) Biotinylated antibody (100x) | 1 | 130 µL |
Avidin-Biotin-Peroxidase Complex (100x) | 1 | 130 µL |
Sample Diluent | 1 | 30mL |
Antibody Diluent | 1 | 12mL |
Avidin-Biotin-Peroxidase Diluent | 1 | 12mL |
Wash Buffer Concentrate (Powder for 1000mL) | 1 | Pack |
Color Developing Reagent | 1 | 10mL |
Stop Solution | 1 | 10mL |
Plate Sealers | 4 | Each |
Reproducibility
| Intra-Assay | Inter-Assay | ||||
Sample ID | 1 | 2 | 3 | 1 | 2 | 3 |
n= | 16 | 16 | 16 | 24 | 24 | 24 |
Mean Measured Concentration (pg/mL) | 11.6 | 45.5 | 188 | 10.3 | 52 | 213 |
Standard Deviation | 0.56 | 2.64 | 11.28 | 0.56 | 3.43 | 15.55 |
Consistency (%CV) | 4.8 | 5.8 | 6 | 5.4 | 6.6 | 7.3 |