Human IL-8 Pre-Coated ELISA Kit
description
The BioGems Human IL-8 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure IL-8 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-8. It contains E. coli-expressed recombinant IL-8. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-8. The kit is analytically validated with ready to use reagents.
Product Details
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Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<1pg/ml
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Range:
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15.6pg/ml-1000pg/ml (cell culture supernates) 7.8pg/ml-500pg/ml (serum,plasma)
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Specificity:
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Natural and recombinant Human IL-8
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Uniprot:
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P10145
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 - 8 °C.
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Components
Description | Quantity | Volume |
Anti-Human IL-8 Pre-coated 96-well strip microplate | 1 | 12 strips of 8 wells |
Human IL-8 Standard | 2 | 10 ng vial |
Human IL-8 Biotinylated antibody (100x) | 1 | 130 µL |
Avidin-Biotin-Peroxidase Complex (100x) | 1 | 130 µL |
Sample Diluent | 1 | 30mL |
Antibody Diluent | 1 | 12mL |
Avidin-Biotin-Peroxidase Diluent | 1 | 12mL |
Wash Buffer Concentrate (Powder for 1000mL) | 1 | Pack |
Color Developing Reagent | 1 | 10mL |
Stop Solution | 1 | 10mL |
Plate Sealers | 4 | Each |
Reproducibility
| Intra-Assay | Inter-Assay | ||||
Sample ID | 1 | 2 | 3 | 1 | 2 | 3 |
n= | 16 | 16 | 16 | 24 | 24 | 24 |
Mean Measured Concentration (pg/mL) | 115 | 357 | 616 | 176 | 392 | 649 |
Standard Deviation | 6.67 | 17.5 | 41.3 | 13.38 | 21.17 | 45.43 |
Consistency (%CV) | 5.8 | 4.9 | 6.7 | 7.6 | 5.4 | 7 |