Human IL-3 Pre-Coated ELISA Kit

Grouped product items
Size Price Qty
1 Kit
$480.00
Catalog Number : BGK08700

description

IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoiesis, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is a species-specific, variably glycosylated cytokine.

The BioGems Human IL-3 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure IL-3 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-3. It contains E. coli-expressed recombinant IL-3. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-3. The kit is analytically validated with ready to use reagents.

Product Details

Assay Type:
Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:
<1pg/ml
Range:
15.6pg/ml-1000pg/ml
Specificity:
Natural and recombinant Human IL-3
Uniprot:
P08700
Interference:
No detectable cross-reactivity with other relevant proteins
Storage Conditions:
Store the unopened product at 2 - 8 °C.

Components

Description

Quantity

Volume

Anti-Human IL-3 Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human IL-3 Standard

2

10 ng vial

Human IL-3 Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

95

323

590

108

372

625

Standard Deviation

4.56

12.6

21.83

8.75

24.18

33.75

Consistency (%CV)

4.8

3.9

3.7

8.1

6.5

5.4

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