Human Growth Hormone Pre-Coated ELISA Kit
description
Growth Hormone (GH), also known as somatotropin, is a pleiotropic cytokine of the hematopoietic growth factor superfamily, which encompasses most cytokines, hematopoietic growth factors, and related receptors, and includes the related growth hormone receptor, prolactin, placental lactogens, proliferins, and somatolactin (SST). GH is primarily recognized for its anabolic role in stimulating the growth and differentiation of muscle, bone, and cartilage. A number of other functions, including immunomodulatory actions, are also attributed to GH, due in part to the pervasive distribution of its receptors, and the indirect effects associated with GH-stimulated production of insulin-like growth factors (IGFs). Occurring predominantly in the somatotropes of the anterior pituitary, whereupon it is stored in secretory granules, production of GH has also been noted in many other tissues, including those of the hematopoietic system. The production and pulsatile release of circulating GH is very tightly regulated by both negative and positive feedback regulations of pituitary and hypothalamic hormones, such as Pituitary-specific Positive Transcription Factor 1 (POU1F1), Growth Hormone Releasing Hormone (GHRH), and somatostatin (SRIF). Deficient production of GH is associated with dwarfism and reduction of lean body mass, while overproduction is associated with acromegaly and gigantism, as well as breast tumor growth.
The BioGems Human Growth Hormone Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human Growth Hormone with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for VEGF. It contains E. coli-expressed recombinant Human Growth Hormone. The detection antibody is a biotinylated goat polyclonal antibody specific for Growth Hormone. The kit is analytically validated with ready to use reagents.
Product Details
Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<2 pg/ml
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Range:
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15.6 pg/mL- 1,000 pg/mL
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Specificity:
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Natural and recombinant Human Growth Hormone
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Uniprot:
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P01241
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 - 8 °C.
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Components
Description |
Quantity |
Volume |
Anti-Human Growth Hormone Pre-coated 96-well strip microplate |
1 |
12 strips of 8 wells |
Human Growth Hormone Standard |
2 |
10 ng vial |
Human Growth Hormone Biotinylated antibody (100x) |
1 |
130 µL |
Avidin-Biotin-Peroxidase Complex (100x) |
1 |
130 µL |
Sample Diluent |
1 |
30mL |
Antibody Diluent |
1 |
12mL |
Avidin-Biotin-Peroxidase Diluent |
1 |
12mL |
Wash Buffer Concentrate (Powder for 1000mL) |
1 |
Pack |
Color Developing Reagent |
1 |
10mL |
Stop Solution |
1 |
10mL |
Plate Sealers |
4 |
Each |
Reproducibility
|
Intra-Assay |
Inter-Assay |
||||
Sample ID |
1 |
2 |
3 |
1 |
2 |
3 |
n= |
16 |
16 |
16 |
24 |
24 |
24 |
Mean Measured Concentration (pg/mL) |
101 |
335 |
618 |
121 |
354 |
596 |
Standard Deviation |
4.44 |
12.06 |
24.10 |
9.922 |
26.55 |
43.51 |
Consistency (%CV) |
4.4 |
3.6 |
3.9 |
8.2 |
7.5 |
7.3 |