Human IL-10 Pre-Coated ELISA Kit
description
IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF-α. Like IL-10, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 is active on murine cells, but murine IL-10 is inactive on human cells.
The BioGems Human IL-10 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human IL-10 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-10. It contains E. coli-expressed recombinant Human IL-10. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-10. The kit is analytically validated with ready to use reagents.
Product Details
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Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<0.5 pg/ml
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Range:
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7.8 pg/mL- 500 pg/mL
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Specificity:
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Natural and recombinant Human IL-10
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Uniprot:
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P22301
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 - 8 °C.
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Components
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Description |
Quantity |
Volume |
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Anti-Human IL-10 Pre-coated 96-well strip microplate |
1 |
12 strips of 8 wells |
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Human IL-10 Standard |
2 |
10 ng vial |
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Human IL-10 Biotinylated antibody (100x) |
1 |
130 µL |
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Avidin-Biotin-Peroxidase Complex (100x) |
1 |
130 µL |
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Sample Diluent |
1 |
30mL |
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Antibody Diluent |
1 |
12mL |
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Avidin-Biotin-Peroxidase Diluent |
1 |
12mL |
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Wash Buffer Concentrate (Powder for 1000mL) |
1 |
Pack |
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Color Developing Reagent |
1 |
10mL |
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Stop Solution |
1 |
10mL |
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Plate Sealers |
4 |
Each |
Reproducibility
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Intra-Assay |
Inter-Assay |
||||
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Sample ID |
1 |
2 |
3 |
1 |
2 |
3 |
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n= |
16 |
16 |
16 |
24 |
24 |
24 |
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Mean Measured Concentration (pg/mL) |
25 |
112 |
280 |
34 |
184 |
312 |
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Standard Deviation |
1.35 |
5.38 |
8.68 |
2.55 |
14.54 |
20.9 |
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Consistency (%CV) |
5.4 |
4.8 |
3.1 |
7.5 |
7.9 |
6.7 |