Viability Dye 506
description
The Viability Dye 506 can be used to exclude non-viable cells in flow cytometry assays. It irreversibly binds to cell surface and intracellular amines. The dye can covalently bind to a higher concentration of amines in cells that have lost plasma membrane integrity, such as those in the late stages of apoptosis or dead cells, and generate a significantly more intense fluorescence signal. The advantage of this viability dye over propidium iodide and 7-AAD, is that the cells it denotes can be washed, fixed, permeabilized and intracellularly stained without losing the fluorescence of the viability staining.
The Viability Dye 506 can be excited by the violet (405nm) laser and has a peak emission of 506nm detectable by the 510/50 band pass filter.
(The 500 tests size will be supplied as 5 x 100 tests.)
Additional Information
Applications:
|
FC
|
Application: | FC |
---|---|
Application Notes:
|
The antibody has been analyzed for quality through the flow cytometric analysis of the relevant cell type. The antibody can be used at less than or equal to 5 μL per test. A test is the amount of antibody required to stain a cell sample in the final volume of 100 μL.
|
Formulation:
|
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
|
References:
|
Perfetto, S. P., Chattopadhyay, P. K., Lamoreaux, L., Nguyen, R., Ambrozak, D., Koup, R. A., & Roederer, M. (2006). Amine reactive dyes: an effective tool to discriminate live and dead cells in polychromatic flow cytometry. Journal of immunological methods, 313(1), 199-208
|