Recombinant Murine Sonic Hedgehog (Shh)
Catalog Number : 315-22
Members of the Hedgehog (Hh) family are highly conserved proteins which are widely represented throughout the animal kingdom. The three known mammalian Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh) are structurally related and share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93% identical). The biologically active form of Hh molecules is obtained by autocatalytic cleavage of their precursor proteins and corresponds to approximately the N-terminal one half of the precursor molecule. Although Hh proteins have unique expression patterns and distinct biological roles within their respective regions of secretion, they use the same signaling pathway and can substitute for each other in experimental systems. Recombinant murine Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the naturally occurring chemically modified Cys residue.
Above 90% as determined by SDS-PAGE Analysis.
Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.5-1.0 μg/ml.
IVIGPGRGF GKRRHPKKL TPLAYKQFI PNVAEKTLG ASGRYEGKI TRNSERFKE LTPNYNPDI IFKDEENTG ADRLMTQRC KDKLNALAI SVMNQWPGV KLRVTEGWD EDGHHSEES LHYEGRAVD ITTSDRDRS KYGMLARLA VEAGFDWVY YESKAHIHC SVKAENSVA AKSGG
|Research Interest:||Stem Cell Research, Apoptosis, Immunology, Bone Biology|
|Protein Cross Reactivity:||NULL|