Recombinant Murine sCD40 Ligand
Catalog Number : 315-15
CD40, a member of the TNF receptor family, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. The soluble form of CD40L is an 18kDa protein comprising the entire TNF homologous region of CD40L and is generated in vivo by an intracellular proteolytic processing of the full length CD40L. Recombinant murine CD40L is a soluble 16.4 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.
Above 98% as determined by SDS-PAGE Analysis.
Determined by its ability to induce TNF-α and MIP-1α production by murine splenocytes. The expected ED50 for this effect is < 0.1 µg/ml.
MQRGDEDPQ IAAHVVSEA NSNAASVLQ WAKKGYYTM KSNLVMLEN GKQLTVKRE GLYYVYTQV TFCSNREPS SQRPFIVGL WLKPSSGSE RILLKAANT HSSSQLCEQ QSVHLGGVF ELQAGASVF VNVTEASQV IHRVGFSSF GLLKL
|Research Interest:||Adaptive Immunity, Apoptosis, Angiogenesis, Inflammation, Proliferation and Cell Cycle|
|Cell Type:||B Cells, Macrophage / Monocyte, T Cells, Dendritic Cells|
|Protein Cross Reactivity:||Human, Rat|