Recombinant Murine Granzyme B
Catalog Number : 140-03
description
Granzyme B is a cysteine protease found in the cytoplasmic granules of cytolytic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme B is required for the induction of target cell lysis, which occurs as part of cell mediated immune responses, and can activate apoptosis in target cells by both caspase dependent and caspase independent mechanisms. Proteolytic cleavage of substrates by Granzyme B takes place primarily after aspartic acid residues. Recombinant murine Granzyme B is a glycosylated 227 amino acid protein, comprising the mature active portion of the murine Granzyme B precursor. The apparent molecular weight is 28.9 kDa by mass spectrometry.
Additional Information
Source: | Baculovirus |
Purity: | Above 98% as determined by SDS-PAGE Analysis. |
Cross Reactivity: | NULL |
Biological Activity | Determined by its ability to cleave a synthetic chromogentic Granzyme B substrate. The expected specific activity, when using the Ac-IEPD-pNA substrate at 250C, is greater than 750 nM/min per μg of enzym |
AA Sequence | IIGGHEVKP HSRPYMALL SIKDQQPEA ICGGFLIRE DFVLTAAHC EGSIINVTL GAHNIKEQE KTQQVIPMV KCIPHPDYN PKTFSNDIM LLKLKSKAK RTRAVRPLN LPRRNVNVK PGDVCYVAG WGRMAPMGK YSNTLQEVE LTVQKDREC ESYFKNRYN KTNQICAGD PKTKRASFR GDSGGPLVC KKVAAGIVS YGYKDGSPP RAFTKVSSF LSWIKKTMK SS |
| Research Interest: | Innate Immunity, Apoptosis, Inflammation |
|---|---|
| Cell Type: | NK and NKT Cells, T Regulatory Cells |
| Application: | FA |
| Protein Cross Reactivity: | NULL |