Recombinant Murine Adiponectin

Grouped product items
SizePrice Qty
5µg
$80.00
25µg
$195.00
100µg
$700.00
500µg
$3,120.00
1mg
$5,200.00
Catalog Number : 315-26

description

Adiponectin is an adipose-derived secreted protein containing 236 amino acid residues.  It is relatively abundant in humans and rodents, accounting for about 0.01% of total plasma protein.  The circulating levels of adiponectin are decreased under conditions of obesity, insulin resistance, and type II diabetes.  Disruption of adiponectin in mice causes insulin resistance and neointimal formation.  Conversely, administration of recombinant adiponectin suppresses hepatic glucose production, and reverses insulin resistance associated with both lipoatrophy and obesity.  The protective role of adiponectin is attributed to its anti-inflammatory properties (e.g. ability to suppress expression of TNF-α and class A scavenger receptor in macrophages). Recombinant adiponectin is a multimeric glycoprotein containing amino acids Val-21 to Asn-247 of the adiponectin precursor protein fused to an N-terminal histidine tag. Recombinant Murine Adiponectin is a multimeric glycoprotein, which has a calculated molecular weight of 25.8 kDa and contains amino acids Val-21 to Asn-247 of the adiponectin precursor protein fused to an N-terminal histidine tag. As a result of glycosylation, Recombinant Murine Adiponectin migrates with an apparent molecular mass of approximately 31-36 kDa by SDS-PAGE gel, under reducing conditions.

Additional Information

Source:
Hi-5 Insect cells
Purity:
Above 95% as determined by SDS-PAGE Analysis.
Cross Reactivity:
NULL
Biological Activity
Determined by a cytotoxic assay using M1 cells.  The ED50 for this effect is 4.0-6.0 μg/ml.
AA Sequence
RGHHHHHHH HVTTTEELA PALVPPPKG TCAGWMAGI PGHPGHNGT PGRDGRDGT PGEKGEKGD AGLLGPKGE TGDVGMTGA EGPRGFPGT PGRKGEPGE AAYVYRSAF SVGLETRVT VPNVPIRFT KIFYNQQNH YDGSTGKFY CNIPGLYYF SYHITVYMK DVKVSLFKK DKAVLFTYD QYQEKNVDQ ASGSVLLHL EVGDQVWLQ VYGDGDHNG LYADNVNDS TFTGFLLYH DTN
Research Interest:Inflammation, Metabolic Regulation and Diabetes
Cell Type:Macrophage / Monocyte
Application:FA
Protein Cross Reactivity:NULL
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