Recombinant Human WNT-1
Catalog Number : 120-17
Wnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, β-catenin. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues. Recombinant human Wnt-1 is a 38.4 kDa, non-glycosylated protein containing 343 amino acid residues.
Above 98% as determined by SDS-PAGE Analysis.
The ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/m
ANSSGRWWG IVNVASSTN LLTDSKSLQ LVLEPSLQL LSRKQRRLI RQNPGILHS VSGGLQSAV RECKWQFRN RRWNCPTAP GPHLFGKIV NRGCRETAF IFAITSAGV THSVARSCS EGSIESCTC DYRRRGPGG PDWHWGGCS DNIDFGRLF GREFVDSGE KGRDLRFLM NLHNNEAGR TTVFSEMRQ ECKCHGMSG SCTVRTCWM RLPTLRAVG DVLRDRFDG ASRVLYGNR GSNRASRAE LLRLEPEDP AHKPPSPHD LVYFEKSPN FCTYSGRLG TAGTAGRAC NSSSPALDG CELLCCGRG HRTRTQRVT ERCNCTFHW CCHVSCRNC THTRVLHEC L
|Research Interest:||Stem Cell Research, Cancer, Inflammation|
|Cell Type:||Stem Cells|
|Protein Cross Reactivity:||Mouse|