Recombinant Human sFas Receptor
Catalog Number : 310-20
Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. The full length Fas (receptor) is a 319 amino acid type I transmembrane protein, which contains a 157 amino acid extracellular domain, a 17 amino acid transmembrane domain, and 145 amino acid cytoplasmic domain. Recombinant human soluble Fas (sFas Receptor) is a 157 amino acid polypeptide (17.6 kDa) corresponding to the TNFR homologous cysteine rich extracellular domain Fas.
Above 98% as determined by SDS-PAGE Analysis.
The ED50 was determined by its ability to inhibit the cytotoxicity of Jurkat cells is between 10-15 µg/ml in the presence of 2ng/ml of hFasL.
MRLSSKSVN AQVTDINSK GLELRKTVT TVETQNLEG LHHDGQFCH KPCPPGERK ARDCTVNGD EPDCVPCQE GKEYTDKAH FSSKCRRCR LCDEGHGLE VEINCTRTQ NTKCRCKPN FFCNSTVCE HCDPCTKCE HGIIKECTL TSNTKCKEE GSRS
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