Recombinant Human sCD100
Catalog Number : 310-29
The Semphorins are a large family of phylogenetically conserved proteins that play a pivotal role in maintaining homeostasis in the immune system. Twenty members of this family have been identified and categorized into eight subclasses based on sequence similarity and distinctive structural features. CD100, also known as Sema4D, is a 150 kDa transmembrane class IV semaphorin. Studies have shown that CD100 can induce monocyte migration, T-cell activation, and B-cell survival, as well as T/B cell and T/DC “cooperation”. The CD100 precursor contains 862 amino acids, including a 21 a.a. signal sequence, a 713 a.a. extracellular domain, a 21 a.a transmembrane sequence, and a 107 a.a cytoplasmic region. The extracellular sequence contains several structural features, including a 479 a.a “sema” domain, a 79 a.a. Ig-like sequence, and a 52 a.a “Plexin-type repeat”. Recombinant soluble CD100 is a 78.9 kDa protein comprising the extracellular domain of CD100 (711 amino acids). SDS-PAGE analysis run under non-reducing conditions shows a mixture of disulfide linked dimer and monomer.
Above 97% as determined by SDS-PAGE Analysis.
Measured by its ability to inhibit chemokine (hMCP-3) induced human monocyte migration.
FAPIPRITW EHREVHLVQ FHEPDIYNY SALLLSEDK DTLYIGARE AVFAVNALN ISEKQHEVY WKVSEDKKA KCAEKGKSK QTECLNYIR VLQPLSATS LYVCGTNAF QPACDHLNL TSFKFLGKN EDGKGRCPF DPAHSYTSV MVDGELYSG TSYNFLGSE PIISRNSSH SPLRTEYAI PWLNEPSFV FADVIRKSP DSPDGEDDR VYFFFTEVS VEYEFVFRV LIPRIARVC KGDQGGLRT LQKKWTSFL KARLICSRP DSGLVFNVL RDVFVLRSP GLKVPVFYA LFTPQLNNV GLSAVCAYN LSTAEEVFS HGKYMQSTT VEQSHTKWV RYNGPVPKP RPGACIDSE ARAANYTSS LNLPDKTLQ FVKDHPLMD DSVTPIDNR PRLIKKDVN YTQIVVDRT QALDGTVYD VMFVSTDRG ALHKAISLE HAVHIIEET QLFQDFEPV QTLLLSSKK GNRFVYAGS NSGVVQAPL AFCGKHGTC EDCVLARDP YCAWSPPTA TCVALHQTE SPSRGLIQE MSGDASVCP DKSKGSYRQ HFFKHGGTA ELKCSQKSN LARVFWKFQ NGVLKAESP KYGLMGRKN LLIFNLSEG DSGVYQCLS EERVKNKTV FQVVAKHVL EVKVVPKPV VAPTLSVVQ TEGSRIATK VLVASTQGS SPPTPAVQA TSSGAITLP PKPAPTGTS CEPKIVINT VPQLHSEKT MYLKSSDNR
|Research Interest:||Innate Immunity, Adaptive Immunity|
|Cell Type:||B Cells, Macrophage / Monocyte, T Cells|
|Protein Cross Reactivity:||NULL|