Recombinant Human HVEM-Fc
Catalog Number : 310-27
HVEM belongs to the TNF Receptor superfamily of transmembrane proteins and plays a role in the activation of T-cells and other lymphocytes. It is expressed in various cells and tissues including spleen, thymus, lung, macrophages, and T-cells. HVEM activation induces a signaling cascade which results in induction of transcription factors NF-kappaB and AP-1. LIGHT (TNFSF14) and TNF-β (TNFSF1) function as the ligands for HVEM, which can also bind specifically to herpes simplex virus glycoprotein D. Soluble HVEM can act as a “receptor decoy” resulting in inhibition of the activity of the HVEM ligands, LIGHT and TNF-β. Recombinant human HVEM-Fc Chimera is a 376 amino acid fusion protein containing an N-terminal domain corresponding to the extracellular region of HVEM and a C-terminal domain corresponding to residues 102 to 330 of human IgG1.
Hi-5 Insect cells
Above 98% as determined by SDS-PAGE Analysis.
Determined by its ability to neutralize 0.25 ng/ml of hTNFβ induced cytotoxicity on murine L929 cells. The expected ED50 for this effect is 1.3-1.9 μg/ml of HVEM-Fc.
LPSCKEDEY PVGSECCPK CSPGYRVKE ACGELTGTV CEPCPPGTY IAHLNGLSK CLQCQMCDP AMGLRASRN CSRTENAVC GCSPGHFCI VQDGDHCAA CRAYATSSP GQRVQKGGT ESQDTLCQN CPPGTFSPN GTLEECQHQ TKRSCDKTH TCPPCPAPE LLGGPSVFL FPPKPKDTL MISRTPEVT CVVVDVSHE DPEVKFNWY VDGVEVHNA KTKPREEQY NSTYRVVSV LTVLHQDWL NGKEYKCKV SNKALPAPI EKTISKAKG QPREPQVYT LPPSRDELT KNQVSLTCL VKGFYPSDI AVEWESNGQ PENNYKTTP PVLDSDGSF FLYSKLTVD KSRWQQGNV FSCSVMHEA LHNHYTQKS LSLSPGK
|Research Interest:||Adaptive Immunity, Apoptosis, Receptors|
|Cell Type:||Macrophage / Monocyte|
|Protein Cross Reactivity:||Mouse|