Recombinant Human TIGAR-TAT
Catalog Number : 150-14T
TIGAR is a p53-inducible enzyme that catalyzes the hydrolysis of fructose-2-6 bisphosphate (F-2-6-BP) to fructose-6-phosphate and inorganic phosphate. F-2-6-BP is a powerful activator of 6-phosphofructose-1 kinase, the rate limiting enzyme of glycolysis. By lowering the intracellular level of F-2-6-BP, TIGAR expression leads to increased glucose processing via the pentose phosphate pathway, the major cellular source for NADPH. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors and other intracellular proteins into primary as well as transformed cells. Recombinant human TIGAR-TAT expressed in E. coli is a 31.6 kDa protein containing 283 amino-acid residues, including the 269 residues of full-length TIGAR fused to a 14-residue C-terminal peptide containing the TAT transduction domain (GGGYGRKKRRQRRR).
Above 95% as determined by SDS-PAGE Analysis.
Pretreatment with TIGAR-TAT for 4 hrs, using a concentration range 0.1-5.0 μg/ml, protects U2OS cells from apoptosis induced by hydrogen peroxide.
ARFALTVVR HGETRFNKE KIIQGQGVD EPLSETGFK QAAAAGIFL NNVKFTHAF SSDLMRTKQ TMHGILERS KFCKDMTVK YDSRLRERK YGVVEGKAL SELRAMAKA AREECPVFT PPGGETLDQ VKMRGIDFF EFLCQLILK EADQKEQFS QGSPSNCLE TSLAEIFPL GKNHSSKVN SDSGIPGLA ASVLVVSHG AYMRSLFDY FLTDLKCSL PATLSRSEL MSVTPNTGM SLFIINFEE GREVKPTVQ CICMNLQDH LNGLTETRG GGYGRKKRR QRRR
|Research Interest:||Apoptosis, Cancer, Proliferation and Cell Cycle|
|Protein Cross Reactivity:||NULL|