Recombinant Human PAF-AH
Catalog Number : 140-10
Platelet Activating Factor (PAF) is a biologically active phospholipid, which exerts primarily proinflammatory activities by specifically signaling through G-protein-coupled receptors on platelets, neutrophils, and monocytes. Platelet Activating Factor Acetylhydrolase (PAF-AH) is a secreted protein that mediates PAF activity by specifically catalyzing hydrolysis of the “sn2” ester bond, resulting in the conversion of PAF to the biologically inactive lyso-PAF. PAF-AH can also interact with LDL particles to induce the hydrolysis of LDL associated, oxidized phospholipids, generating lysophosphatidylcholine (lyso-PC) and other lysophospholipids. Recombinant PAF-AH is a 420 amino acid glycoprotein which migrates with an apparent molecular mass of 47-55 kDa by SDS-PAGE analysis.
Above 95% as determined by SDS-PAGE Analysis.
Measured by its ability to cleave a PAF analog in a chromogenic substrate linked assay. At a PAF-AH concentration of 10.0 μg/ml, 50% cleavage was achieved at an incubation time of approximately 2 minutes.
FDWQYINPV AHMKSSAWV NKIQVLMAA ASFGQTKIP RGNGPYSVG CTDLMFDHT NKGTFLRLY YPSQDNDRL DTLWIPNKE YFWGLSKFL GTHWLMGNI LRLLFGSMT TPANWNSPL RPGEKYPLV VFSHGLGAF RTLYSAIGI DLASHGFIV AAVEHRDRS ASATYYFKD QSAAEIGDK SWLYLRTLK QEEETHIRN EQVRQRAKE CSQALSLIL DIDHGKPVK NALDLKFDM EQLKDSIDR EKIAVIGHS FGGATVIQT LSEDQRFRC GIALDAWMF PLGDEVYSR IPQPLFFIN SEYFQYPAN IIKMKKCYS PDKERKMIT IRGSVHQNF ADFTFATGK IIGHMLKLK GDIDSNVAI DLSNKASLA FLQKHLGLH KDFDQWDCL IEGDDENLI PGTNINTTN QHIMLQNSS GIEKYN
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