Recombinant Human Osteopontin
Catalog Number : 120-35
Osteopontin is a secreted glycoprotein that functions as a ligand to αvβ3 integrin and possibly other receptors. It binds tightly to hydroxyapatite and can act as a structural component of the extracellular mineralized matrix. Osteopontin is initially secreted as a 298 amino acid protein, which is subject to multiple post-translational modifications including glycosylation, phosphorylation, and specific proteolytic cleavages into various smaller molecular weight fragments. Osteopontin is expressed in a wide range of cells and tissues including osteoblasts, various tumor cell lines, extraosseous cells in the inner ear, brain, kidney, deciduum, placenta and odontoblasts. In addition to its involvement in mineralized matrix formation, Osteopontin can also function as a cytokine that stimulates the release of IFNγ and IL-12, while inhibiting the production of IL-10. Recombinant human Osteopontin is a 298 amino acid protein, which, due to glycosylation, migrates at an apparent molecular weight of 60.0-65.0 kDa by SDS-PAGE analysis under reducing conditions.
Above 97% as determined by SDS-PAGE Analysis.
Determined by its ability to chemoattract human monocytes using a concentration range of 10.0-100.0 ng/ml.
IPVKQADSG SSEEKQLYN KYPDAVATW LNPDPSQKQ NLLAPQNAV SSEETNDFK QETLPSKSN ESHDHMDDM DDEDDDDHV DSQDSIDSN DSDDVDDTD DSHQSDESH HSDESDELV TDFPTDLPA TEVFTPVVP TVDTYDGRG DSVVYGLRS KSKKFRRPD IQYPDATDE DITSHMESE ELNGAYKAI PVAQDLNAP SDWDSRGKD SYETSQLDD QSAETHSHK QSRLYKRKA NDESNEHSD VIDSQELSK VSREFHSHE FHSHEDMLV VDPKSKEED KHLKFRISH ELDSASSEV N
|Research Interest:||Innate Immunity, Cancer, Bone Biology|
|Cell Type:||Macrophage / Monocyte, T Cells|
|Protein Cross Reactivity:||NULL|