Catalog Number : 500-M85
Produced in mice using highly pure recombinant human β-NGF as the immunizing antigen. This IgG2bK antibody was purified from cell culture by Protein G affinity chromatography.
|Application:||ELISA, Western Blot, Neutralization, Immunohistochemistry|
Anti-Human β-NGF (BioGems Catalog #450-01)
This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma. The recommended concentration is 2.5 µg/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 9.0 buffer is recommended. See photo(s). Optimal concentrations and conditions may vary.
Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
Information and photo are courtesy of Flagship Biosciences, LLC – Histology Services.
To yield one-half maximal inhibition [ND50] of the biological activity of Human β-NGF (10.0 ng/ml), a concentration of 2.7 - 4.0 μg/ml of this antibody is required.
In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 200 pg/ml of recombinant Human β-NGF when used with BioGems' biotinylated antigen affinity purified anti-Human β-NGF (60-247BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
To detect Human β-NGF by Western Blot analysis this antibody can be used at a concentration of 4.0-8.0 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human β-NGF is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.