Recombinant Human TNF-β (E.coli-derived)
Catalog Number : 300-01B
TNF-β is a potent mediator of inflammatory and immune responses. It belongs to the TNF family of ligands and signals through TNFR1 and TNFR2. TNF-β is produced by activated T and B lymphocytes, and has similar activities to TNF-α. Like TNF-α, TNF-β is involved in the regulation of various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNF-β is secreted as a soluble polypeptide, but can form heterotrimers with lymphotoxin-β, which effectively anchors the TNF-β to the cell surface. TNF-β is cytotoxic to a wide range of tumor cells. Recombinant human TNF-β is a secreted 171 amino acid protein (18.6 kDa) which forms an active non-disulfide linked homotrimer structure in solution.
Above 98% as determined by SDS-PAGE Analysis.
The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin-D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.
MLPGVGLTP SAAQTARQH PKMHLAHST LKPAAHLIG DPSKQNSLL WRANTDRAF LQDGFSLSN NSLLVPTSG IYFVYSQVV FSGKAYSPK ATSSPLYLA HEVQLFSSQ YPFHVPLLS SQKMVYPGL QEPWLHSMY HGAAFQLTQ GDQLSTHTD GIPHLVLSP STVFFGAFA L
|Research Interest:||Stem Cell Research, Apoptosis, Immunology, Angiogenesis, Neurosciences|
|Cell Type:||B Cells, T Cells|
|Protein Cross Reactivity:||Mouse|