Animal-Free Recombinant Murine TNF-α
TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant murine TNF-α is a soluble 156 amino acid protein (17.3 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein. Manufactured using all non-animal reagents.
Above 98% as determined by SDS-PAGE Analysis.
The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
MLRSSSQNS SDKPVAHVV ANHQVEEQL EWLSQRANA LLANGMDLK DNQLVVPAD GLYLVYSQV LFKGQGCPD YVLLTHTVS RFAISYQEK VNLLSAVKS PCPKDTPEG AELKPWYEP IYLGGVFQL EKGDQLSAE VNLPKYLDF AESGQVYFG VIAL
|Research Interest:||Innate Immunity, Adaptive Immunity, Stem Cell Research, Apoptosis, Cancer, Angiogenesis, Neurosciences, Inflammation, Metabolic Regulation and Diabetes|
|Cell Type:||B Cells, Macrophage / Monocyte, T Cells|
|Protein Cross Reactivity:||Monkey, Rat|