Recombinant Murine IL-12
Catalog Number : 210-12
description
IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-γ production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue type mast cells. Among its biological activities IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-γ producing Th1 cells. Recombinant murine IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (506 total amino acid residues).
Additional Information
|
Source:
|
CHO cells
|
|
Purity:
|
Above 98% as determined by SDS-PAGE Analysis.
|
|
Cross Reactivity:
|
Human
|
|
Biological Activity
|
Assay #1: The ED50 was determined by the stimulation of IFN-gamma production by murine splenocytes co-stimulated with IL-18 is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 uni
|
|
AA Sequence
|
p35 Subunit:< /B>RVIPVS GPARCLSQS RNLLKTTDD MVKTAREKL KHYSCTAED IDHEDITRD QTSTLKTCL PLELHKNES CLATRETSS TTRGSCLPP QKTSLMMTL CLGSIYEDL KMYQTEFQA INAALQNHN HQQIILDKG MLVAIDELM QSLNHNGET LRQKPPVGE ADPYRVKMK LCILLHAFS TRVVTINRV MGYLSSA p 40Subunit :MWEL EKDVYVVEV DWTPDAPGE TVNLTCDTP EEDDITWTS DQRHGVIGS GKTLTITVK EFLDAGQYT CHKGGETLS HSHLLLHKK ENGIWSTEI LKNFKNKTF LKCEAPNYS GRFTCSWLV QRNMDLKFN IKSSSSSPD SRAVTCGMA SLSAEKVTL DQRDYEKYS VSCQEDVTC PTAEETLPI ELALEARQQ NKYENYSTS FFIRDIIKP DPPKNLQMK PLKNSQVEV SWEYPDSWS TPHSYFSLK FFVRIQRKK EKMKETEEG CNQKGAFLV EKTSTEVQC KGGNVCVQA QDRYYNSSC SKWACVPCR VRS |
| Research Interest: | Innate Immunity, Stem Cell Research, Immunology, Cancer, Angiogenesis, Inflammation |
|---|---|
| Cell Type: | Macrophage / Monocyte, NK and NKT Cells, T Cells, Dendritic Cells |
| Application: | FA |
| Protein Cross Reactivity: | Human |