Recombinant Human IL-21
Catalog Number : 200-21
IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjuction) with IL-4 of IgG production, and induction of apoptotic effects in naïve B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the γ chain (γc) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Recombinant human IL-21 is a 15.4 kDa protein consisting of 132 amino acid residues.
Above 98% as determined by SDS-PAGE Analysis.
Assay #1:Determined by its ability to proliferate activated B cells.
Assay #2:Determined by its ability to induce human CD40L-activated naïve B cells to undergo Ig isotype switching to IgG, using an IL-
MQDRHMIRM RQLIDIVDQ LKNYVNDLV PEFLPAPED VETNCEWSA FSCFQKAQL KSANTGNNE RIINVSIKK LKRKPPSTN AGRRQKHRL TCPSCDSYE KKPPKEFLE RFKSLLQKM IHQHLSSRT HGSEDS
|Research Interest:||Adaptive Immunity, Stem Cell Research, Apoptosis, Immunology, Cancer|
|Cell Type:||B Cells, T Cells|
|Protein Cross Reactivity:||Mouse|