Recombinant Human IFN-γ
Catalog Number : 300-02
IFN-γ is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-γ signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. Additionally, IFN-γ stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-γ species-specific and is biologically active only in human and primate cells. Recombinant human IFN-γ is a 16.8 kDa protein containing 144 amino acid residues.
Above 98% as determined by SDS-PAGE Analysis.
Assay #1: Determined by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 5.0-10.0 ng/ml.
Assay #2: The ED50 was determined by a cytotoxicity assay using HT-29
MQDPYVKEA ENLKKYFNA GHSDVADNG TLFLGILKN WKEESDRKI MQSQIVSFY FKLFKNFKD DQSIQKSVE TIKEDMNVK FFNSNKKKR DDFEKLTNY SVTDLNVQR KAIHELIQV MAELSPAAK TGKRKRSQM LFQGRRASQ
|Research Interest:||Innate Immunity, Stem Cell Research, Immunology, Cancer, Angiogenesis, Neurosciences, Metabolic Regulation and Diabetes|
|Cell Type:||B Cells, Macrophage / Monocyte, NK and NKT Cells, T Regulatory Cells|
|Protein Cross Reactivity:||Monkey|