Animal-Free Recombinant Human IFN-γ
description
IFN-γ is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-γ signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. Additionally, IFN-γ stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-γ species-specific and is biologically active only in human and primate cells. Recombinant human IFN-γ is a 16.8 kDa protein containing 144 amino acid residues. Manufactured using all non-animal reagents.
Additional Information
Source: | E. Coli |
Purity: | Above 98% as determined by SDS-PAGE Analysis. |
Cross Reactivity: | Monkey |
Biological Activity | Assay #1: Determined by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 5.0-10.0 ng/ml. Assay #2: The ED50 was determined by a cytotoxicity assay using HT-29 |
AA Sequence | MQDPYVKEA ENLKKYFNA GHSDVADNG TLFLGILKN WKEESDRKI MQSQIVSFY FKLFKNFKD DQSIQKSVE TIKEDMNVK FFNSNKKKR DDFEKLTNY SVTDLNVQR KAIHELIQV MAELSPAAK TGKRKRSQM LFQGRRASQ |
Research Interest: | Innate Immunity, Stem Cell Research, Immunology, Cancer, Angiogenesis, Neurosciences, Metabolic Regulation and Diabetes |
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Cell Type: | B Cells, Macrophage / Monocyte, NK and NKT Cells, T Regulatory Cells |
Application: | FA |
Protein Cross Reactivity: | Monkey |